Hotspot

trnK-AAA-rps16

LSC · 4326-4928 · 603 bp

Back to Cymatocarpus

Pi 0.0357
MarkerSeek score 69.90
Primer available yes
Label trnK-AAA-rps16

Feature Metadata

label_name
trnK-AAA-rps16
feature_type
igs
region
LSC
length_bp
603
variable_sites
32
pi
0.0357
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-AAA-rps16_p1 CCTTTCAGGATCAGTCGTGG CCCAATGAGCCGTTTATCGA 55.0 50.0 58.0 58.1 965-1057 bp 1.0 83.6
trnK-AAA-rps16_p2 CCTTTCAGGATCAGTCGTGG TCCCAATGAGCCGTTTATCG 55.0 50.0 58.0 58.1 966-1058 bp 1.0 83.6
trnK-AAA-rps16_p3 TCAGGATCAGTCGTGGTCTT CCCAATGAGCCGTTTATCGA 50.0 50.0 58.1 58.1 961-1053 bp 1.0 83.2
trnK-AAA-rps16_p4 TCAGGATCAGTCGTGGTCTT TCCCAATGAGCCGTTTATCG 50.0 50.0 58.1 58.1 962-1054 bp 1.0 83.2
trnK-AAA-rps16_p5 TTCAGGATCAGTCGTGGTCT CCCAATGAGCCGTTTATCGA 50.0 50.0 58.1 58.1 962-1054 bp 1.0 83.2