Hotspot

ycf90

Genome · 57498-58868 · 1371 bp

Back to Cyclotella

Pi 0.149

MarkerSeek score 80.30

Primer available yes

Label ycf90

Feature Metadata

label_name: ycf90
feature_type: gene
region: Genome
length_bp: 1371
variable_sites: 408
pi: 0.149
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf90_p1	TGTTGTATTGGGCTTGCTGT	ACGCAAGATCGTCAACAAGT	45.0	45.0	58.0	58.1	2560-2576 bp	0.333333	56.6
ycf90_p2	TGTTGTATTGGGCTTGCTGT	GTTTTTGGAACACCACGTCC	45.0	50.0	58.0	57.8	2593-2609 bp	0.333333	56.1
ycf90_p3	TGTTGTATTGGGCTTGCTGT	TTTTTGGAACACCACGTCCT	45.0	45.0	58.0	57.9	2608 bp	0.166667	45.6
ycf90_p4	AACTTGCCAAAGGTATGGGG	TTTTTGGAACACCACGTCCT	50.0	45.0	58.1	57.9	2706 bp	0.166667	45.1
ycf90_p5	AACTTGCCAAAGGTATGGGG	ACGCAAGATCGTCAACAAGT	50.0	45.0	58.1	58.1	2674 bp	0.166667	45.1