Hotspot

rpl6

Genome · 97926-98525 · 600 bp

Back to Cyclotella

Pi 0.176

MarkerSeek score 84.50

Primer available yes

Label rpl6

Feature Metadata

label_name: rpl6
feature_type: gene
region: Genome
length_bp: 600
variable_sites: 212
pi: 0.176
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl6_p1	GTAATGACAGGTAGGCAGGC	TTATAGGACTCCCCGCGAAT	55.0	50.0	58.0	58.0	1180 bp	0.166667	46.3
rpl6_p2	GCAGGCGATGTCTAATGGAA	TTATAGGACTCCCCGCGAAT	50.0	50.0	58.0	58.0	1166 bp	0.166667	46.3
rpl6_p3	AGTAAACCTGGTTTGCGTGT	TTATAGGACTCCCCGCGAAT	45.0	50.0	58.0	58.0	1273 bp	0.166667	46.2
rpl6_p4	GTAATGACAGGTAGGCAGGC	TAAGCGTGGTCTTTCGACTG	55.0	50.0	58.0	57.9	843 bp	0.166667	45.8
rpl6_p5	GCAGGCGATGTCTAATGGAA	TAAGCGTGGTCTTTCGACTG	50.0	50.0	58.0	57.9	829 bp	0.166667	45.8