Hotspot

rpl5

Genome · 96752-97474 · 723 bp

Back to Cyclotella

Pi 0.106

MarkerSeek score 82.70

Primer available yes

Label rpl5

Feature Metadata

label_name: rpl5
feature_type: gene
region: Genome
length_bp: 723
variable_sites: 163
pi: 0.106
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl5_p1	GTGTTTTTGGTCCTGTTGCC	ACACGCAAACCAGGTTTACT	50.0	45.0	58.1	58.0	1333-1372 bp	0.333333	58.6
rpl5_p2	GTGTTTTTGGTCCTGTTGCC	GACTGATTGCCCTCTCCATG	50.0	55.0	58.1	58.0	1688 bp	0.166667	45.9
rpl5_p3	GTGTTTTTGGTCCTGTTGCC	GCCTGCCTACCTGTCATTAC	50.0	55.0	58.1	58.0	1465 bp	0.166667	45.8
rpl5_p4	GTGTTTTTGGTCCTGTTGCC	TTCCATTAGACATCGCCTGC	50.0	50.0	58.1	58.0	1479 bp	0.166667	45.8
rpl5_p5	GTGTTTTTGGTCCTGTTGCC	GATCGACTGATTGCCCTCTC	50.0	55.0	58.1	57.9	1692 bp	0.166667	45.0