Hotspot

rpl4

Genome · 91234-91887 · 654 bp

Back to Cyclotella

Pi 0.202

MarkerSeek score 83.20

Primer available yes

Label rpl4

Feature Metadata

label_name: rpl4
feature_type: gene
region: Genome
length_bp: 654
variable_sites: 251
pi: 0.202
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl4_p1	TTGTGGAGTGACGTTTCGTT	TCTGAGAATGCTGAAAGGGC	45.0	50.0	58.0	57.9	1266 bp	0.166667	45.6
rpl4_p2	TTGTGGAGTGACGTTTCGTT	TGAGAATGCTGAAAGGGCTC	45.0	50.0	58.0	57.9	1264 bp	0.166667	45.6
rpl4_p3	TTGTGGAGTGACGTTTCGTT	CTGAGAATGCTGAAAGGGCT	45.0	50.0	58.0	57.9	1265 bp	0.166667	45.6
rpl4_p4	TTGTGGAGTGACGTTTCGTT	CTCCGCCACGATGTCTAATC	45.0	55.0	58.0	58.2	1367 bp	0.166667	44.7
rpl4_p5	GAGCTCCTGGTTCAATTGGT	TGAGAATGCTGAAAGGGCTC	50.0	50.0	57.8	57.9	1476 bp	0.166667	44.1