Hotspot

rpl3

Genome · 90340-91020 · 681 bp

Back to Cyclotella

Pi 0.108

MarkerSeek score 81.60

Primer available yes

Label rpl3

Feature Metadata

label_name: rpl3
feature_type: gene
region: Genome
length_bp: 681
variable_sites: 153
pi: 0.108
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl3_p1	GGGTTGTTCGAACACCATCT	AACGAAACGTCACTCCACAA	50.0	45.0	58.1	58.0	1078 bp	0.166667	45.7
rpl3_p2	GGGTTGTTCGAACACCATCT	AGCACGACCTGTTCCTTTTT	50.0	45.0	58.1	57.9	1490 bp	0.166667	45.4
rpl3_p3	GGGTTGTTCGAACACCATCT	TTTGTTTTTGGCAGCACGTT	50.0	40.0	58.1	57.9	1693 bp	0.166667	45.1
rpl3_p4	GGGTTGTTCGAACACCATCT	TTTTGTTTTTGGCAGCACGT	50.0	40.0	58.1	57.9	1694 bp	0.166667	45.1
rpl3_p5	GGGTTGTTCGAACACCATCT	TAAACCTCCGCACATGTCAG	50.0	50.0	58.1	57.9	1667 bp	0.166667	45.1