Hotspot

rpl13

Genome · 103194-103682 · 489 bp

Back to Cyclotella

Pi 0.2

MarkerSeek score 81.20

Primer available yes

Label rpl13

Feature Metadata

label_name: rpl13
feature_type: gene
region: Genome
length_bp: 489
variable_sites: 192
pi: 0.2
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl13_p1	TGGTCGTAAATCGGCAGATG	TTCGTGCTTTCCGTAAACCA	50.0	45.0	58.1	58.0	1169-1315 bp	0.333333	64.4
rpl13_p2	TGGTCGTAAATCGGCAGATG	CTTCCCATTTCCCGGAACAA	50.0	50.0	58.1	58.1	1026 bp	0.166667	45.5
rpl13_p3	TGGTCGTAAATCGGCAGATG	TTGGCCAGTTAATCCACCAC	50.0	50.0	58.1	58.1	1173 bp	0.166667	45.5
rpl13_p4	ACTCGACAATTTGGACGCTT	TTCGTGCTTTCCGTAAACCA	45.0	45.0	58.1	58.0	1908 bp	0.166667	45.3
rpl13_p5	TGGTCGTAAATCGGCAGATG	TCTGCTTGGCCAGTTAATCC	50.0	50.0	58.1	57.9	1178 bp	0.166667	45.1