Hotspot

dnaK

Genome · 88284-90071 · 1788 bp

Back to Cyclotella

Pi 0.158

MarkerSeek score 81.20

Primer available yes

Label dnaK

Feature Metadata

label_name: dnaK
feature_type: gene
region: Genome
length_bp: 1788
variable_sites: 552
pi: 0.158
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
dnaK_p1	CCCGCAAAAGTAACACGTTC	TGAGTCATAGGTCCTCTTGCA	50.0	47.6	58.0	58.2	2778-2826 bp	0.333333	51.2
dnaK_p2	TTCTCGGGCTATTCTTGCAC	TGAGTCATAGGTCCTCTTGCA	50.0	47.6	58.0	58.2	2935-2983 bp	0.333333	51.1
dnaK_p3	ATCCCGCAAAAGTAACACGT	TGAGTCATAGGTCCTCTTGCA	45.0	47.6	58.1	58.2	2780-2828 bp	0.333333	50.5
dnaK_p4	TGCTGGAATTAATGGTCCCG	TGAGTCATAGGTCCTCTTGCA	50.0	47.6	57.9	58.2	2941 bp	0.166667	36.8
dnaK_p5	GCTGGAATTAATGGTCCCGT	TGAGTCATAGGTCCTCTTGCA	50.0	47.6	57.9	58.2	2940 bp	0.166667	36.8