Hotspot

clpC

Genome · 108012-111206 · 3195 bp

Back to Cyclotella

Pi 0.243

MarkerSeek score 78.00

Primer available yes

Label clpC

Feature Metadata

label_name: clpC
feature_type: gene
region: Genome
length_bp: 3195
variable_sites: 1350
pi: 0.243
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpC_p1	TAGCACGCAAAAGTATCGCT	AGCTAATGCAAGTCCTTCCG	45.0	50.0	58.0	58.0	1010 bp	0.166667	46.4
clpC_p2	GCAGTAAATGAAGCCCGAGA	AGCTAATGCAAGTCCTTCCG	50.0	50.0	58.0	58.0	855 bp	0.166667	46.2
clpC_p3	TGAAGCCCGAGACATGAATG	AGCTAATGCAAGTCCTTCCG	50.0	50.0	58.0	58.0	847 bp	0.166667	46.2
clpC_p4	AGCAGTAAATGAAGCCCGAG	AGCTAATGCAAGTCCTTCCG	50.0	50.0	58.0	58.0	856 bp	0.166667	46.2
clpC_p5	GAAGCCCGAGACATGAATGT	AGCTAATGCAAGTCCTTCCG	50.0	50.0	58.0	58.0	846 bp	0.166667	46.2