Hotspot

ccsA

Genome · 81818-82873 · 1056 bp

Back to Cyclotella

Pi 0.302

MarkerSeek score 82.00

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: Genome
length_bp: 1056
variable_sites: 569
pi: 0.302
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	CGAGTTGTTTCAGCCCCTAA	TCTGCTGTTCGTCGAGAAAC	50.0	50.0	57.8	58.2	1541-1583 bp	0.333333	56.9
ccsA_p2	CGGCTTTACATCCATCCCAA	CTGCTGTTCGTCGAGAAACT	50.0	50.0	57.9	58.2	1467 bp	0.166667	44.4
ccsA_p3	CGGCTTTACATCCATCCCAA	GTCTGCTGTTCGTCGAGAAA	50.0	50.0	57.9	58.2	1469 bp	0.166667	44.4
ccsA_p4	CGGCTTTACATCCATCCCAA	TCTGCTGTTCGTCGAGAAAC	50.0	50.0	57.9	58.2	1468 bp	0.166667	44.4
ccsA_p5	CGAGTTGTTTCAGCCCCTAA	CTGCTGTTCGTCGAGAAACT	50.0	50.0	57.8	58.2	1582 bp	0.166667	43.4