Hotspot

thiS-trnQ-UUG

Genome · 85284-86146 · 863 bp

Back to Cyclotella

Pi 0.333
MarkerSeek score 72.50
Primer available yes
Label thiS-trnQ-UUG

Feature Metadata

label_name
thiS-trnQ-UUG
feature_type
igs
region
Genome
length_bp
863
variable_sites
419
pi
0.333
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.50
markerseek_score
72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
thiS-trnQ-UUG_p1 TTTGGGTCAATCCAGGCAAA CTTTGACTCCGCCATTCGTA 45.0 50.0 57.9 58.0 1423 bp 0.166667 45.9
thiS-trnQ-UUG_p2 TTTGGGTCAATCCAGGCAAA TTTGACTCCGCCATTCGTAG 45.0 50.0 57.9 58.0 1422 bp 0.166667 45.9
thiS-trnQ-UUG_p3 TTTTGGGTCAATCCAGGCAA CTTTGACTCCGCCATTCGTA 45.0 50.0 57.9 58.0 1424 bp 0.166667 45.9
thiS-trnQ-UUG_p4 TTTTGGGTCAATCCAGGCAA TTTGACTCCGCCATTCGTAG 45.0 50.0 57.9 58.0 1423 bp 0.166667 45.9
thiS-trnQ-UUG_p5 TTTTTGGGTCAATCCAGGCA TTTGACTCCGCCATTCGTAG 45.0 50.0 57.9 58.0 1424 bp 0.166667 45.9