Hotspot

ycf89-trnP-UGG

Genome · 128597-129082 · 486 bp

Back to Cyclostephanos

Pi 0.105

MarkerSeek score 79.40

Primer available yes

Label ycf89-trnP-UGG

Feature Metadata

label_name: ycf89-trnP-UGG
feature_type: igs
region: Genome
length_bp: 486
variable_sites: 50
pi: 0.105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf89-trnP-UGG_p1	ATACATCGCGGCACAATTCT	AGGATTTGAACCTACGGCAC	45.0	50.0	58.0	57.9	974 bp	0.5	58.8
ycf89-trnP-UGG_p2	GATCACGTGCCTCGAAATCT	AGGATTTGAACCTACGGCAC	50.0	50.0	58.1	57.9	695 bp	0.5	58.5
ycf89-trnP-UGG_p3	AATTGATCACGTGCCTCGAA	AGGATTTGAACCTACGGCAC	45.0	50.0	57.9	57.9	699 bp	0.5	58.4
ycf89-trnP-UGG_p4	ATTGATCACGTGCCTCGAAA	AGGATTTGAACCTACGGCAC	45.0	50.0	57.9	57.9	698 bp	0.5	58.4
ycf89-trnP-UGG_p5	TTGATCACGTGCCTCGAAAT	AGGATTTGAACCTACGGCAC	45.0	50.0	57.9	57.9	697 bp	0.5	58.4