Hotspot

rns-ycf89

Genome · 127081-127630 · 550 bp

Back to Cyclostephanos

Pi 0.134

MarkerSeek score 83.30

Primer available yes

Label rns-ycf89

Feature Metadata

label_name: rns-ycf89
feature_type: igs
region: Genome
length_bp: 550
variable_sites: 70
pi: 0.134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rns-ycf89_p1	CATTGCGGAAAATTCCCCAC	AGAATTGTGCCGCGATGTAT	50.0	45.0	58.0	58.0	1434-1458 bp	1.0	86.5
rns-ycf89_p2	TATTGCTCCGTCAGGCTTTC	AGAATTGTGCCGCGATGTAT	50.0	45.0	58.0	58.0	1457-1481 bp	1.0	86.2
rns-ycf89_p3	CAACCCATAGGCAGTCTTCC	AGAATTGTGCCGCGATGTAT	55.0	45.0	58.0	58.0	1486-1510 bp	1.0	86.0
rns-ycf89_p4	ATTGCGGAAAATTCCCCACT	AGAATTGTGCCGCGATGTAT	45.0	45.0	58.1	58.0	1433-1457 bp	1.0	86.0
rns-ycf89_p5	CTTGCATCCTCCGTCTTACC	AGAATTGTGCCGCGATGTAT	55.0	45.0	58.1	58.0	1585-1609 bp	1.0	85.4