Hotspot

ccs1-ycf46

Genome · 115272-115278 · 7 bp

Back to Cyclostephanos

Pi 0.143

MarkerSeek score 79.20

Primer available yes

Label ccs1-ycf46

Feature Metadata

label_name: ccs1-ycf46
feature_type: igs
region: Genome
length_bp: 7
variable_sites: 1
pi: 0.143
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccs1-ycf46_p1	ACTTAAAGCGGCAATCATCA	AAAATTGGGCAGTCTCTGGG	40.0	50.0	55.7	58.1	147 bp	1.0	61.8
ccs1-ycf46_p2	ACTTAAAGCGGCAATCATCAA	AAAATTGGGCAGTCTCTGGG	38.1	50.0	56.5	58.1	147 bp	1.0	60.0
ccs1-ycf46_p3	ACTTAAAGCGGCAATCATCAAT	AAAATTGGGCAGTCTCTGGG	36.4	50.0	57.0	58.1	147 bp	1.0	56.1
ccs1-ycf46_p4	ACTTAAAGCGGCAATCATCA	GCGAATTCGTCTTGCTTCTT	40.0	45.0	55.7	57.1	128 bp	1.0	55.4
ccs1-ycf46_p5	ACTTAAAGCGGCAATCATCA	GGCGAATTCGTCTTGCTTCT	40.0	50.0	55.7	58.9	129 bp	1.0	55.1