Hotspot

ycf89-rns

Genome · 66606-67155 · 550 bp

Back to Cyclostephanos

Pi 0.134

MarkerSeek score 83.30

Primer available yes

Label ycf89-rns

Feature Metadata

label_name: ycf89-rns
feature_type: igs
region: Genome
length_bp: 550
variable_sites: 70
pi: 0.134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf89-rns_p1	AGAATTGTGCCGCGATGTAT	CATTGCGGAAAATTCCCCAC	45.0	50.0	58.0	58.0	1434-1458 bp	1.0	86.5
ycf89-rns_p2	AGAATTGTGCCGCGATGTAT	TATTGCTCCGTCAGGCTTTC	45.0	50.0	58.0	58.0	1457-1481 bp	1.0	86.2
ycf89-rns_p3	AGAATTGTGCCGCGATGTAT	CAACCCATAGGCAGTCTTCC	45.0	55.0	58.0	58.0	1486-1510 bp	1.0	86.0
ycf89-rns_p4	AGAATTGTGCCGCGATGTAT	ATTGCGGAAAATTCCCCACT	45.0	45.0	58.0	58.1	1433-1457 bp	1.0	86.0
ycf89-rns_p5	AGAATTGTGCCGCGATGTAT	CTTGCATCCTCCGTCTTACC	45.0	55.0	58.0	58.1	1585-1609 bp	1.0	85.4