Hotspot

petA-chlI

Genome · 44606-45179 · 574 bp

Back to Cyclostephanos

Pi 0.163

MarkerSeek score 82.10

Primer available yes

Label petA-chlI

Feature Metadata

label_name: petA-chlI
feature_type: igs
region: Genome
length_bp: 574
variable_sites: 90
pi: 0.163
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-chlI_p1	GCCGTGGACAAGTTTATCCA	GCAAAAGCACATGCAGCATA	50.0	45.0	58.2	58.0	1137-1144 bp	1.0	88.1
petA-chlI_p2	GCCGTGGACAAGTTTATCCA	GGTATGCATGCCGAAATTCG	50.0	50.0	58.2	58.0	1401-1408 bp	1.0	87.3
petA-chlI_p3	GCCGTGGACAAGTTTATCCA	TCGTCATCCTGCTCGATTTG	50.0	50.0	58.2	58.1	1483-1490 bp	1.0	86.7
petA-chlI_p4	GCCGTGGACAAGTTTATCCA	CAATTCGTCATCCTGCTCGA	50.0	50.0	58.2	58.1	1487-1494 bp	1.0	86.6
petA-chlI_p5	GCCGTGGACAAGTTTATCCA	GAGCGAATTGTAGATGCCCA	50.0	50.0	58.2	58.0	1269 bp	0.5	58.2