Hotspot

rpoB-rps20

Genome · 38763-39199 · 437 bp

Back to Cyclostephanos

Pi 0.133

MarkerSeek score 75.60

Primer available yes

Label rpoB-rps20

Feature Metadata

label_name: rpoB-rps20
feature_type: igs
region: Genome
length_bp: 437
variable_sites: 58
pi: 0.133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-rps20_p1	GTTTCTCCCTGCCAATTCCA	AAGTTCCATTCTTTGCGGGT	50.0	45.0	58.1	58.0	1702-1747 bp	1.0	84.4
rpoB-rps20_p2	GTTTCTCCCTGCCAATTCCA	AGTTCCATTCTTTGCGGGTT	50.0	45.0	58.1	58.0	1701-1746 bp	1.0	84.4
rpoB-rps20_p3	TGGTTTCTCCCTGCCAATTC	AAGTTCCATTCTTTGCGGGT	50.0	45.0	58.1	58.0	1704-1749 bp	1.0	84.4
rpoB-rps20_p4	TGGTTTCTCCCTGCCAATTC	AGTTCCATTCTTTGCGGGTT	50.0	45.0	58.1	58.0	1703-1748 bp	1.0	84.4
rpoB-rps20_p5	GTTTCTCCCTGCCAATTCCA	TGCGGGTTATTTCGACGATT	50.0	45.0	58.1	58.0	1689-1734 bp	1.0	84.4