Hotspot

ycf45-ffs

Genome · 22905-23575 · 671 bp

Back to Cyclostephanos

Pi 0.203

MarkerSeek score 80.50

Primer available yes

Label ycf45-ffs

Feature Metadata

label_name: ycf45-ffs
feature_type: igs
region: Genome
length_bp: 671
variable_sites: 134
pi: 0.203
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf45-ffs_p1	CCCGCATTTCAACTTGCAAT	TCCACTGCTCTACCAACTGA	45.0	50.0	57.9	58.0	1418-1420 bp	1.0	87.9
ycf45-ffs_p2	CCCGCATTTCAACTTGCAAT	ATCGCATCAATAAACGGCCT	45.0	45.0	57.9	58.0	1706-1708 bp	1.0	87.6
ycf45-ffs_p3	CCCGCATTTCAACTTGCAAT	AACTGAGCTATCCGGGCTTA	45.0	50.0	57.9	58.2	1404-1406 bp	1.0	86.3
ycf45-ffs_p4	CCCGCATTTCAACTTGCAAT	CCCCCTAGGCACAGTAGAAT	45.0	55.0	57.9	58.2	1539 bp	0.5	57.8
ycf45-ffs_p5	CCCGCATTTCAACTTGCAAT	ACTGAGCTATCCGGGCTTAA	45.0	50.0	57.9	58.2	1405 bp	0.5	57.7