Hotspot

psbX-rbcS

Genome · 7614-8162 · 549 bp

Back to Cyclostephanos

Pi 0.134

MarkerSeek score 82.30

Primer available yes

Label psbX-rbcS

Feature Metadata

label_name: psbX-rbcS
feature_type: igs
region: Genome
length_bp: 549
variable_sites: 70
pi: 0.134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbX-rbcS_p1	TGCTGTCAAACTCGCGATAA	GCTCGTAAATCATGCGCTTC	45.0	50.0	57.9	58.0	801-818 bp	1.0	89.6
psbX-rbcS_p2	TGCTGTCAAACTCGCGATAA	TCGGTGGTGGTACTATTGGT	45.0	50.0	57.9	58.1	1309-1326 bp	1.0	85.6
psbX-rbcS_p3	TGCTGTCAAACTCGCGATAA	GAAGCTCGTAAATCATGCGC	45.0	50.0	57.9	58.0	804 bp	0.5	59.3
psbX-rbcS_p4	TGCTGTCAAACTCGCGATAA	ATCCGCATCCTCGTAACAAC	45.0	50.0	57.9	58.1	893 bp	0.5	58.9
psbX-rbcS_p5	CGTCTCTTGCAACTTCAGGT	GCTCGTAAATCATGCGCTTC	50.0	50.0	58.1	58.0	1160 bp	0.5	58.9