Hotspot

rpl32-trnL-UAG

SSC · 114850-115742 · 893 bp

Back to Cyclorhiza

Pi 0.0121
MarkerSeek score 61.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
893
variable_sites
19
pi
0.0121
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGAAATCTCTTTCTACCGGA AGCGTGTCTACCGATTTCAC 47.6 50.0 56.8 58.0 985-1048 bp 1.0 66.6
rpl32-trnL-UAG_p2 GGGAAATCTCTTTCTACCGGA CAGCGTGTCTACCGATTTCA 47.6 50.0 56.8 58.0 986-1049 bp 1.0 66.6
rpl32-trnL-UAG_p3 AGGGAAATCTCTTTCTACCGG AGCGTGTCTACCGATTTCAC 47.6 50.0 56.8 58.0 986-1049 bp 1.0 66.5
rpl32-trnL-UAG_p4 AGGGAAATCTCTTTCTACCGG CAGCGTGTCTACCGATTTCA 47.6 50.0 56.8 58.0 987-1050 bp 1.0 66.5
rpl32-trnL-UAG_p5 AGGGAAATCTCTTTCTACCGGA AGCGTGTCTACCGATTTCAC 45.5 50.0 58.3 58.0 986-1049 bp 1.0 65.8