Hotspot

petA-psbJ

LSC · 64183-64930 · 748 bp

Back to Cyclorhiza

Pi 0.0218

MarkerSeek score 59.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 748
variable_sites: 29
pi: 0.0218
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CAGGGCCAGAACTTCTTGTT	AACTGGCCGTTTTGATCCTT	50.0	45.0	58.0	58.0	1559-1717 bp	1.0	81.6
petA-psbJ_p2	CAGGGCCAGAACTTCTTGTT	ACTGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	1558-1716 bp	1.0	81.6
petA-psbJ_p3	TCAGAGCTATCGCCCCAATA	AACTGGCCGTTTTGATCCTT	50.0	45.0	58.0	58.0	1879-2037 bp	1.0	81.5
petA-psbJ_p4	TCAGAGCTATCGCCCCAATA	ACTGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	1878-2036 bp	1.0	81.5
petA-psbJ_p5	CGGATTTGGTCAGGGAGATG	ACTGGCCGTTTTGATCCTTT	55.0	45.0	58.0	58.0	1482-1640 bp	1.0	81.5