Pi
0.0169
MarkerSeek score
61.10
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 839-870 bp | 1.0 | 81.8 |
| trnT-UGU-trnL-UAA_p2 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 871-902 bp | 1.0 | 80.8 |
| trnT-UGU-trnL-UAA_p3 | GCGATGCTCTAACCTCTGAG | ATTAAGTCCGCAGCGTCTAC | 55.0 | 50.0 | 57.9 | 57.8 | 857-888 bp | 1.0 | 80.1 |
| trnT-UGU-trnL-UAA_p4 | GGAATCGAACCGATGACCAT | ATTAAGTCCGCAGCGTCTAC | 50.0 | 50.0 | 57.8 | 57.8 | 889-920 bp | 1.0 | 79.2 |
| trnT-UGU-trnL-UAA_p5 | ATGCGATGCTCTAACCTCTG | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.5 | 58.0 | 841-872 bp | 1.0 | 78.7 |