Hotspot

trnT-GGU-psbD

LSC · 32671-34090 · 1420 bp

Back to Cyclorhiza

Pi 0.0149

MarkerSeek score 66.00

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1420
variable_sites: 37
pi: 0.0149
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	AATTGAATCAAACGGGCCCT	AGACCGGACCATCCTACAAA	45.0	50.0	58.1	58.1	1181-1622 bp	1.0	85.0
trnT-GGU-psbD_p2	ATTGAATCAAACGGGCCCTT	AGACCGGACCATCCTACAAA	45.0	50.0	58.1	58.1	1180-1621 bp	1.0	85.0
trnT-GGU-psbD_p3	AACGGGCCCTTTTAACTCAG	AGACCGGACCATCCTACAAA	50.0	50.0	58.1	58.1	1171-1612 bp	1.0	84.8
trnT-GGU-psbD_p4	GCCCTTTTAACTCAGCGGTA	AGACCGGACCATCCTACAAA	50.0	50.0	57.9	58.1	1166-1607 bp	1.0	84.7
trnT-GGU-psbD_p5	ATTGAATCAAACGGGCCCTT	GAGCAATAGACCGGACCATC	45.0	55.0	58.1	57.8	1187-1628 bp	1.0	84.2