Hotspot

atpI-rps2

LSC · 16070-16322 · 253 bp

Back to Cyclorhiza

Pi 0.0283

MarkerSeek score 65.40

Primer available yes

Label atpI-rps2

Feature Metadata

label_name: atpI-rps2
feature_type: igs
region: LSC
length_bp: 253
variable_sites: 14
pi: 0.0283
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-rps2_p1	TGGAACAGGATAGAACATTCA	CAGCCAACGATGACGCTATA	38.1	50.0	54.3	57.9	351-378 bp	1.0	48.0
atpI-rps2_p2	TGGAACAGGATAGAACATTCA	TGTGAAGGGCGTTCTAGCTA	38.1	50.0	54.3	58.4	289-316 bp	1.0	47.7
atpI-rps2_p3	TGGAACAGGATAGAACATTCA	TTTGTGAAGGGCGTTCTAGC	38.1	50.0	54.3	58.5	291-318 bp	1.0	47.6
atpI-rps2_p4	TGGAACAGGATAGAACATTCA	GCAATTTGTGAAGGGCGTTC	38.1	50.0	54.3	58.6	295-322 bp	1.0	47.6
atpI-rps2_p5	TGGAACAGGATAGAACATTCAT	CAGCCAACGATGACGCTATA	36.4	50.0	54.9	57.9	351-378 bp	1.0	47.0