Hotspot

trnK-UUU-rps16

LSC · 4319-5200 · 882 bp

Back to Cyclorhiza

Pi 0.0201
MarkerSeek score 61.70
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
882
variable_sites
30
pi
0.0201
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAGCCGAGTACTCTACCGTT AAAGGCGGAGGTTTTTAAGT 50.0 40.0 58.2 55.5 937-963 bp 1.0 61.7
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT AAAGGCGGAGGTTTTTAAGT 50.0 40.0 58.2 55.5 938-964 bp 1.0 61.6
trnK-UUU-rps16_p3 CCGCACTTAAAAGCCGAGTA AAAGGCGGAGGTTTTTAAGT 50.0 40.0 58.3 55.5 947-973 bp 1.0 60.7
trnK-UUU-rps16_p4 AAAGCCGAGTACTCTACCGT AAAAGGCGGAGGTTTTTAAGT 50.0 38.1 58.2 56.3 939-965 bp 1.0 60.1
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT AAAAGGCGGAGGTTTTTAAGT 50.0 38.1 58.2 56.3 938-964 bp 1.0 60.1