Hotspot

rpl16

LSC · 81129-82509 · 1381 bp

Back to Cyclopogon

Pi 0.0234

MarkerSeek score 61.60

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1381
variable_sites: 32
pi: 0.0234
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CGAGCAATCGCACCAAAAAT	GCAGGGTTCCCTTACAAACA	45.0	50.0	58.0	58.0	2097-2142 bp	1.0	81.6
rpl16_p2	CGCACCAAAAATCCGAGTTC	GCAGGGTTCCCTTACAAACA	50.0	50.0	58.0	58.0	2089-2134 bp	1.0	81.4
rpl16_p3	TCTCAATTCCCGAGCAATCG	GCAGGGTTCCCTTACAAACA	50.0	50.0	58.1	58.0	2107-2152 bp	1.0	81.2
rpl16_p4	CGAGCAATCGCACCAAAAAT	CCGGTTTTGAAGGAATTGCC	45.0	50.0	58.0	57.9	2469-2514 bp	1.0	81.0
rpl16_p5	ACTTCGGGTGCTAATGACAC	GCAGGGTTCCCTTACAAACA	50.0	50.0	57.9	58.0	2148-2193 bp	1.0	81.0