Hotspot

infA

LSC · 79672-79905 · 234 bp

Back to Cyclopogon

Pi 0

MarkerSeek score 19.20

Primer available yes

Label infA

Feature Metadata

label_name: infA
feature_type: gene
region: LSC
length_bp: 234
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 19.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA_p1	TCATAACCTCTGCTCGTTGC	GGCGGAATGGGGATTGTAAT	50.0	50.0	58.0	58.0	1106-1130 bp	1.0	81.7
infA_p2	CCCACTTCTACGAATGGCTC	GGCGGAATGGGGATTGTAAT	55.0	50.0	58.1	58.0	1162-1186 bp	1.0	81.3
infA_p3	TCATAACCTCTGCTCGTTGC	TTTAGGCGGAATGGGGATTG	50.0	50.0	58.0	57.9	1110-1134 bp	1.0	81.2
infA_p4	TCATAACCTCTGCTCGTTGC	TTAAAGCGTATCAGCCGACC	50.0	50.0	58.0	58.1	1172-1196 bp	1.0	81.1
infA_p5	TCGAATTAGATCCCCCACGA	GGCGGAATGGGGATTGTAAT	50.0	50.0	57.9	58.0	495 bp	1.0	80.6