Hotspot

rrn5S-rrn4.5S

IRa · 130613-130821 · 209 bp

Back to Cyclopogon

Pi 0.0478

MarkerSeek score 77.80

Primer available yes

Label rrn5S-rrn4.5S

Feature Metadata

label_name: rrn5S-rrn4.5S
feature_type: igs
region: IRa
length_bp: 209
variable_sites: 10
pi: 0.0478
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5S-rrn4.5S_p1	TATCGTCACCGCAGTAGAGT	CAGCTGAGGCATCCTAACAG	50.0	55.0	58.0	58.0	324 bp	1.0	81.8
rrn5S-rrn4.5S_p2	AGTATCGTCACCGCAGTAGA	GTCAAGTGGAAGTGCAGTGA	50.0	50.0	58.0	58.0	352 bp	1.0	81.6
rrn5S-rrn4.5S_p3	TATCGTCACCGCAGTAGAGT	GTCAAGTGGAAGTGCAGTGA	50.0	50.0	58.0	58.0	350 bp	1.0	81.6
rrn5S-rrn4.5S_p4	AGTATCGTCACCGCAGTAGA	CGAGCCGCTTATCATTACGA	50.0	50.0	58.0	58.0	377 bp	1.0	81.5
rrn5S-rrn4.5S_p5	TATCGTCACCGCAGTAGAGT	CGAGCCGCTTATCATTACGA	50.0	50.0	58.0	58.0	375 bp	1.0	81.5