Hotspot

ndhF-rpl32

SSC · 112424-113108 · 685 bp

Back to Cyclopogon

Pi 0.0353

MarkerSeek score 69.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 685
variable_sites: 22
pi: 0.0353
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACACATTCCCACAAGTTCCC	TCCACCGGACAATCAAAAAGT	50.0	42.9	58.0	58.1	1154-1225 bp	1.0	77.3
ndhF-rpl32_p2	CACATTCCCACAAGTTCCCA	TCCACCGGACAATCAAAAAGT	50.0	42.9	58.0	58.1	1153-1224 bp	1.0	77.3
ndhF-rpl32_p3	GGAAGCGGAAAAAGGGGTAT	TCCACCGGACAATCAAAAAGT	50.0	42.9	57.9	58.1	733-804 bp	1.0	76.9
ndhF-rpl32_p4	GAAAGCCCACATACGACGAA	TCCACCGGACAATCAAAAAGT	50.0	42.9	58.3	58.1	809-880 bp	1.0	75.5
ndhF-rpl32_p5	TTTTTGACAAGCACTTGCCG	TCCACCGGACAATCAAAAAGT	45.0	42.9	58.1	58.1	1285 bp	0.5	50.9