Hotspot

infA-rps8

LSC · 79906-80019 · 114 bp

Back to Cyclopogon

Pi 0.0526

MarkerSeek score 71.90

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: LSC
length_bp: 114
variable_sites: 6
pi: 0.0526
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	ACCGAACTCGAAACATACCG	TGGGGGAGAAATTTTGTGTT	50.0	40.0	58.0	55.3	218 bp	1.0	60.6
infA-rps8_p2	ACCGAACTCGAAACATACCG	TTGGGGGAGAAATTTTGTGT	50.0	40.0	58.0	55.3	219 bp	1.0	60.6
infA-rps8_p3	CCGAACTCGAAACATACCGT	TGGGGGAGAAATTTTGTGTT	50.0	40.0	58.0	55.3	217 bp	1.0	60.6
infA-rps8_p4	CCGAACTCGAAACATACCGT	TTGGGGGAGAAATTTTGTGT	50.0	40.0	58.0	55.3	218 bp	1.0	60.6
infA-rps8_p5	ACTCGAAACATACCGTTGGG	TGGGGGAGAAATTTTGTGTT	50.0	40.0	57.9	55.3	213 bp	1.0	60.0