Hotspot

petD-rpoA

LSC · 77595-78000 · 406 bp

Back to Cyclopogon

Pi 0.0335
MarkerSeek score 73.70
Primer available yes
Label petD-rpoA

Feature Metadata

label_name
petD-rpoA
feature_type
igs
region
LSC
length_bp
406
variable_sites
13
pi
0.0335
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petD-rpoA_p1 CGTCGTCCAGTAGCTACAAC CCCCCTTTTCCTTTCAAGACA 55.0 47.6 58.1 58.0 562-579 bp 1.0 75.1
petD-rpoA_p2 TTTTTGATCGGTACCGCAGT CCCCCTTTTCCTTTCAAGACA 45.0 47.6 57.8 58.0 538-555 bp 1.0 74.8
petD-rpoA_p3 TCGTCGTCCAGTAGCTACAA CCCCCTTTTCCTTTCAAGACA 50.0 47.6 58.2 58.0 563-580 bp 1.0 74.5
petD-rpoA_p4 TTCGTCGTCCAGTAGCTACA CCCCCTTTTCCTTTCAAGACA 50.0 47.6 58.2 58.0 564-581 bp 1.0 74.5
petD-rpoA_p5 CATTTCGTCGTCCAGTAGCT CCCCCTTTTCCTTTCAAGACA 50.0 47.6 57.7 58.0 567-584 bp 1.0 73.7