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clpP-psbB

LSC · 71415-72143 · 729 bp

Back to Cyclopogon

Pi 0.0533

MarkerSeek score 85.30

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 729
variable_sites: 33
pi: 0.0533
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 85.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGCACTATACGTCAACCCAA	GGCCCAACCAGAAACTAGAG	50.0	55.0	58.0	57.9	798-907 bp	1.0	86.2
clpP-psbB_p2	CGCACTATACGTCAACCCAA	TGGACAGAAAGCAATCGACC	50.0	50.0	58.0	58.2	764-873 bp	1.0	85.7
clpP-psbB_p3	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	716-825 bp	1.0	85.5
clpP-psbB_p4	CGCACTATACGTCAACCCAA	CAATCGACCGGGATCATTCA	50.0	50.0	58.0	57.8	753-862 bp	1.0	85.4
clpP-psbB_p5	ACCCAAACTGCATCTTCCTC	GGCCCAACCAGAAACTAGAG	50.0	55.0	57.8	57.9	784-893 bp	1.0	84.6