Hotspot

trnP-UGG-psaJ

LSC · 65767-66134 · 368 bp

Back to Cyclopogon

Pi 0.0485

MarkerSeek score 78.70

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 368
variable_sites: 16
pi: 0.0485
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	AAAACAAACGCGCTACCAAG	AGGGCATCTGGAAAGAAACG	45.0	50.0	57.9	58.2	502-513 bp	1.0	84.2
trnP-UGG-psaJ_p2	AAAACAAACGCGCTACCAAG	GGGCATCTGGAAAGAAACGA	45.0	50.0	57.9	58.2	501-512 bp	1.0	84.2
trnP-UGG-psaJ_p3	CAAAACAAACGCGCTACCAA	AGGGCATCTGGAAAGAAACG	45.0	50.0	58.2	58.2	503-514 bp	1.0	83.8
trnP-UGG-psaJ_p4	CAAAACAAACGCGCTACCAA	GGGCATCTGGAAAGAAACGA	45.0	50.0	58.2	58.2	502-513 bp	1.0	83.7
trnP-UGG-psaJ_p5	TTGTACCCAAAACAAACGCG	AGGGCATCTGGAAAGAAACG	45.0	50.0	57.8	58.2	510-521 bp	1.0	83.6