Hotspot

petG-trnW-CCA

LSC · 65338-65458 · 121 bp

Back to Cyclopogon

Pi 0.0579
MarkerSeek score 73.00
Primer available yes
Label petG-trnW-CCA

Feature Metadata

label_name
petG-trnW-CCA
feature_type
igs
region
LSC
length_bp
121
variable_sites
7
pi
0.0579
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petG-trnW-CCA_p1 ATTTACAATACAGGCGCGGT AGCGCTCTTAGTTCAGTTCG 45.0 50.0 58.0 58.0 234-235 bp 1.0 82.0
petG-trnW-CCA_p2 ATTTACAATACAGGCGCGGT CAAAACCCGATGTCGTAGGT 45.0 50.0 58.0 57.9 198-199 bp 1.0 81.8
petG-trnW-CCA_p3 CGGTGATCAGTTGGACCTTT AGCGCTCTTAGTTCAGTTCG 50.0 50.0 57.8 58.0 218-219 bp 1.0 81.0
petG-trnW-CCA_p4 CGGTGATCAGTTGGACCTTT CAAAACCCGATGTCGTAGGT 50.0 50.0 57.8 57.9 182-183 bp 1.0 80.9
petG-trnW-CCA_p5 ATTTACAATACAGGCGCGGT CGCTCTTAGTTCAGTTCGGT 45.0 50.0 58.0 57.7 232-233 bp 1.0 79.4