Hotspot

rbcL-accD

LSC · 55937-56728 · 792 bp

Back to Cyclopogon

Pi 0.0307

MarkerSeek score 67.90

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 792
variable_sites: 24
pi: 0.0307
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CTAGCAAATGGAGCCCTGAG	ACAACATCGAATTGAACCAACA	55.0	36.4	58.0	57.1	906-915 bp	1.0	59.3
rbcL-accD_p2	CTAGCAAATGGAGCCCTGAG	AGACAACATCGAATTGAACCAAC	55.0	39.1	58.0	57.9	908-917 bp	1.0	57.7
rbcL-accD_p3	TGAAGCTAGCAAATGGAGCC	ACAACATCGAATTGAACCAACA	50.0	36.4	58.2	57.1	911-920 bp	1.0	57.6
rbcL-accD_p4	CGCTGCTTGTGAAGTATGGA	ACAACATCGAATTGAACCAACA	50.0	36.4	58.3	57.1	881-890 bp	1.0	57.5
rbcL-accD_p5	CTAGCAAATGGAGCCCTGAG	GACAACATCGAATTGAACCAACA	55.0	39.1	58.0	58.1	907-916 bp	1.0	57.4