Hotspot

trnF-GAA-ndhJ

LSC · 47240-47876 · 637 bp

Back to Cyclopogon

Pi 0.0349

MarkerSeek score 67.10

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 637
variable_sites: 22
pi: 0.0349
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAATTCAAGATGCTCATTGA	50.0	31.8	58.1	54.4	693-694 bp	1.0	43.3
trnF-GAA-ndhJ_p2	AATGGTCGGGATAGCTCAGT	TGAAATTCAAGATGCTCATTGA	50.0	31.8	58.2	54.4	736-737 bp	1.0	43.2
trnF-GAA-ndhJ_p3	ATGGTCGGGATAGCTCAGTT	TGAAATTCAAGATGCTCATTGA	50.0	31.8	58.2	54.4	735-736 bp	1.0	43.2
trnF-GAA-ndhJ_p4	TCGGGATAGCTCAGTTGGTA	TGAAATTCAAGATGCTCATTGA	50.0	31.8	57.6	54.4	731-732 bp	1.0	43.2
trnF-GAA-ndhJ_p5	TGATGCAGGTAAATGGTCGG	TGAAATTCAAGATGCTCATTGA	50.0	31.8	58.0	54.4	747-748 bp	1.0	43.1