Hotspot

trnT-GGU-psbD

LSC · 30909-31905 · 997 bp

Back to Cyclopogon

Pi 0.0271
MarkerSeek score 61.00
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
997
variable_sites
27
pi
0.0271
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GCCCTTTTAACTCAGCGGTA CTACCAACGGCTATAGTCAT 50.0 45.0 57.9 54.1 1089-1095 bp 1.0 49.8
trnT-GGU-psbD_p2 GGCGTAAGTCATCGGTTCAA CTACCAACGGCTATAGTCAT 50.0 45.0 58.3 54.1 1052-1058 bp 1.0 48.5
trnT-GGU-psbD_p3 AAGGCCCTTTTAACTCAGCG CTACCAACGGCTATAGTCAT 50.0 45.0 58.5 54.1 1092-1098 bp 1.0 46.9
trnT-GGU-psbD_p4 GCCATGGTAAGGCGTAAGTC CTACCAACGGCTATAGTCAT 55.0 45.0 58.7 54.1 1062-1068 bp 1.0 45.1
trnT-GGU-psbD_p5 AACTCAGCGGTAGAGTAACG CTACCAACGGCTATAGTCAT 50.0 45.0 57.1 54.1 1081-1087 bp 1.0 43.4