Hotspot

trnE-UUC-trnT-GGU

LSC · 30528-30836 · 309 bp

Back to Cyclopogon

Pi 0.0404

MarkerSeek score 71.50

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 309
variable_sites: 12
pi: 0.0404
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	413-502 bp	1.0	82.8
trnE-UUC-trnT-GGU_p2	CGTTGCCTCCTTGAAAGAGA	TACCGCTGAGTTAAAAGGGC	50.0	50.0	57.8	57.9	378-467 bp	1.0	82.4
trnE-UUC-trnT-GGU_p3	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	391-480 bp	1.0	82.1
trnE-UUC-trnT-GGU_p4	CCTTTCGTGATCCACTACCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.7	58.0	452-541 bp	1.0	81.3
trnE-UUC-trnT-GGU_p5	CTTTCGTGATCCACTACCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.7	58.0	451-540 bp	1.0	81.3