Hotspot

rps12-trnQ-UUG

LSC · 68477-70904 · 2428 bp

Back to Cyclocodon

Pi 0.014

MarkerSeek score 50.70

Primer available yes

Label rps12-trnQ-UUG

Feature Metadata

label_name: rps12-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 2428
variable_sites: 34
pi: 0.014
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-trnQ-UUG_p1	TGACCCTGTTAGTCCGTTCT	GGGTTTTGATCCCGGCATAT	50.0	50.0	58.0	58.0	2897 bp	1.0	81.1
rps12-trnQ-UUG_p2	CTGAACGAGTCGCACATACA	GGGTTTTGATCCCGGCATAT	50.0	50.0	58.0	58.0	2602 bp	1.0	81.0
rps12-trnQ-UUG_p3	TTATGAGGGTTGGCTGGGTA	GGGTTTTGATCCCGGCATAT	50.0	50.0	58.0	58.0	2919 bp	1.0	80.9
rps12-trnQ-UUG_p4	CTGAACGAGTCGCACATACA	AGATATTGGCGAGCTGTTGG	50.0	50.0	58.0	58.0	2804 bp	0.5	59.5
rps12-trnQ-UUG_p5	AGCGGGGGATTTTGAAACAT	GGGTTTTGATCCCGGCATAT	45.0	50.0	58.1	58.0	2542 bp	0.5	59.5