Hotspot

trnV-UAC-ndhC

LSC · 48315-49180 · 866 bp

Back to Cyclocodon

Pi 0.0104
MarkerSeek score 64.70
Primer available yes
Label trnV-UAC-ndhC

Feature Metadata

label_name
trnV-UAC-ndhC
feature_type
igs
region
LSC
length_bp
866
variable_sites
9
pi
0.0104
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-UAC-ndhC_p1 GGTTCGAATCCGTATAGCCC CTGGTCCTTTGCTAATGGGG 55.0 55.0 57.9 58.2 1003 bp 1.0 78.3
trnV-UAC-ndhC_p2 GGTTCGAATCCGTATAGCCC TCCTTTGCTAATGGGGGCTA 55.0 50.0 57.9 58.4 999 bp 1.0 76.9
trnV-UAC-ndhC_p3 GGTTCGAATCCGTATAGCCC CCTTTGCTAATGGGGGCTAA 55.0 50.0 57.9 57.5 998 bp 1.0 76.5
trnV-UAC-ndhC_p4 CGGTTCGAATCCGTATAGCC CTGGTCCTTTGCTAATGGGG 55.0 55.0 58.3 58.2 1004 bp 1.0 76.4
trnV-UAC-ndhC_p5 GGTTCGAATCCGTATAGCCC GGGCTAAAACTCCCGAAAGA 55.0 50.0 57.9 57.5 985 bp 1.0 76.2