Hotspot

rpoB-rpoC1

LSC · 34831-34859 · 29 bp

Back to Cyclocodon

Pi 0.0345

MarkerSeek score 72.30

Primer available yes

Label rpoB-rpoC1

Feature Metadata

label_name: rpoB-rpoC1
feature_type: igs
region: LSC
length_bp: 29
variable_sites: 1
pi: 0.0345
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-rpoC1_p1	ACGATCTTTGGCTCTGGAAC	TTTGTCACCTCGCCAACTAG	50.0	50.0	57.9	57.8	221 bp	1.0	78.2
rpoB-rpoC1_p2	ACGATCTTTGGCTCTGGAAC	AGGCAGGATTTTATTGGCCC	50.0	50.0	57.9	58.2	192 bp	1.0	78.0
rpoB-rpoC1_p3	CGACTGCTTGTTCGAGAACT	TTTGTCACCTCGCCAACTAG	50.0	50.0	58.2	57.8	241 bp	1.0	77.2
rpoB-rpoC1_p4	TCGACTGCTTGTTCGAGAAC	TTTGTCACCTCGCCAACTAG	50.0	50.0	58.2	57.8	242 bp	1.0	77.2
rpoB-rpoC1_p5	CGACTGCTTGTTCGAGAACT	AGGCAGGATTTTATTGGCCC	50.0	50.0	58.2	58.2	212 bp	1.0	76.9