Hotspot

trnG-GCC-trnfM-CAU

LSC · 21051-21225 · 175 bp

Back to Cyclocodon

Pi 0.04

MarkerSeek score 75.50

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 175
variable_sites: 7
pi: 0.04
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	ATGCGGATATGGTCGAATGG	CTTGAGGTCACCGGTTCAAA	50.0	50.0	57.9	58.0	282 bp	1.0	80.9
trnG-GCC-trnfM-CAU_p2	ATGCGGATATGGTCGAATGG	AGTTCTTCTCTTAAGCGCGG	50.0	50.0	57.9	58.0	337 bp	1.0	80.8
trnG-GCC-trnfM-CAU_p3	ATGCGGATATGGTCGAATGG	GTTCTTCTCTTAAGCGCGGA	50.0	50.0	57.9	58.0	336 bp	1.0	80.8
trnG-GCC-trnfM-CAU_p4	ATGCGGATATGGTCGAATGG	TCTCTTAAGCGCGGAGTAGA	50.0	50.0	57.9	58.0	331 bp	1.0	80.6
trnG-GCC-trnfM-CAU_p5	TCTTTGCCAAGGAGAAGACG	AGTTCTTCTCTTAAGCGCGG	50.0	50.0	57.8	58.0	308 bp	1.0	80.4