Hotspot

trnY-GUA-trnE-UUC

LSC · 15358-15416 · 59 bp

Back to Cyclocodon

Pi 0.0339

MarkerSeek score 66.70

Primer available yes

Label trnY-GUA-trnE-UUC

Feature Metadata

label_name: trnY-GUA-trnE-UUC
feature_type: igs
region: LSC
length_bp: 59
variable_sites: 2
pi: 0.0339
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnE-UUC_p1	CCAGCGTAGACATATTGCCA	AAGTATGCCCCCATCGTCTA	50.0	50.0	57.8	57.9	202 bp	1.0	78.1
trnY-GUA-trnE-UUC_p2	ACCAGCGTAGACATATTGCC	AAGTATGCCCCCATCGTCTA	50.0	50.0	57.8	57.9	203 bp	1.0	78.1
trnY-GUA-trnE-UUC_p3	CCAGCGTAGACATATTGCCA	TCTCTTTCAAGGAGGCAACG	50.0	50.0	57.8	57.8	167 bp	1.0	77.7
trnY-GUA-trnE-UUC_p4	ACCAGCGTAGACATATTGCC	TCTCTTTCAAGGAGGCAACG	50.0	50.0	57.8	57.8	168 bp	1.0	77.7
trnY-GUA-trnE-UUC_p5	CGAATTTACAGTCCGTCCCC	AAGTATGCCCCCATCGTCTA	55.0	50.0	58.4	57.9	181 bp	1.0	77.3