Hotspot

atpH-trnD-GUC

LSC · 14055-15090 · 1036 bp

Back to Cyclocodon

Pi 0.0126

MarkerSeek score 66.10

Primer available yes

Label atpH-trnD-GUC

Feature Metadata

label_name: atpH-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1036
variable_sites: 13
pi: 0.0126
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-trnD-GUC_p1	ATAACGGAAGCGGCAGAAAT	TTCAATTGGTCAGAGCACCG	45.0	50.0	58.0	58.5	1131-1132 bp	1.0	76.9
atpH-trnD-GUC_p2	AGCCAATCCAGCAGCAATAA	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.9	58.5	1147-1148 bp	1.0	76.0
atpH-trnD-GUC_p3	AATAACGGAAGCGGCAGAAA	TTCAATTGGTCAGAGCACCG	45.0	50.0	58.2	58.5	1132-1133 bp	1.0	75.7
atpH-trnD-GUC_p4	GCCAATCCAGCAGCAATAAC	TTCAATTGGTCAGAGCACCG	50.0	50.0	57.8	58.5	1146-1147 bp	1.0	75.4
atpH-trnD-GUC_p5	TCCAGCAGCAATAACGGAAG	TTCAATTGGTCAGAGCACCG	50.0	50.0	58.3	58.5	1141-1142 bp	1.0	75.1