Hotspot

trnQ-UUG-psbK

LSC · 7143-7692 · 550 bp

Back to Cyclocodon

Pi 0.0389

MarkerSeek score 67.30

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 550
variable_sites: 21
pi: 0.0389
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	ATATGCCGGGATCAAAACCC	CGAAAACTTACAGCGGCTTG	50.0	50.0	58.0	58.0	776-779 bp	1.0	83.4
trnQ-UUG-psbK_p2	TTAGTCCAACCAAGCCAACC	CGAAAACTTACAGCGGCTTG	50.0	50.0	58.0	58.0	1351-1354 bp	1.0	82.7
trnQ-UUG-psbK_p3	ATATGCCGGGATCAAAACCC	CTTCACGTCCAGGATTACGG	50.0	55.0	58.0	58.1	1336-1339 bp	1.0	82.0
trnQ-UUG-psbK_p4	ATATGCCGGGATCAAAACCC	AAAAGGAAAAGGGCCGGAAT	50.0	45.0	58.0	58.0	958 bp	0.5	59.8
trnQ-UUG-psbK_p5	TTAGTCCAACCAAGCCAACC	AAAAGGAAAAGGGCCGGAAT	50.0	45.0	58.0	58.0	1533 bp	0.5	59.7