Hotspot

rps16-trnQ-UUG

LSC · 5118-7070 · 1953 bp

Back to Cyclocodon

Pi 0.0159

MarkerSeek score 52.80

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1953
variable_sites: 31
pi: 0.0159
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	TGACTTGTGTTGGATTGGCA	GGGTTTTGATCCCGGCATAT	45.0	50.0	57.9	58.0	2410 bp	0.5	59.4
rps16-trnQ-UUG_p2	ACTTGTGTTGGATTGGCACT	GGGTTTTGATCCCGGCATAT	45.0	50.0	57.9	58.0	2408 bp	0.5	59.4
rps16-trnQ-UUG_p3	TGACTTGTGTTGGATTGGCA	AGATATTGGCGAGCTGTTGG	45.0	50.0	57.9	58.0	2612 bp	0.5	59.2
rps16-trnQ-UUG_p4	ACTTGTGTTGGATTGGCACT	AGATATTGGCGAGCTGTTGG	45.0	50.0	57.9	58.0	2610 bp	0.5	59.1
rps16-trnQ-UUG_p5	TGACTTGTGTTGGATTGGCA	TTGGCAAACGGACGTTTTTC	45.0	45.0	57.9	58.1	2596 bp	0.5	58.8