Hotspot

trnK-UUU-rps16

LSC · 4308-4841 · 534 bp

Back to Cyclocodon

Pi 0.0282
MarkerSeek score 60.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
534
variable_sites
15
pi
0.0282
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT TTCTTGAAAGGGGTGCTCAG 50.0 50.0 58.2 57.7 677-678 bp 1.0 78.9
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT TTCTTGAAAGGGGTGCTCAG 50.0 50.0 58.2 57.7 676-677 bp 1.0 78.9
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT GGAGTCTAGTTCAGCGTCAC 50.0 55.0 58.2 57.7 1177-1178 bp 1.0 78.4
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT GGAGTCTAGTTCAGCGTCAC 50.0 55.0 58.2 57.7 1176-1177 bp 1.0 78.4
trnK-UUU-rps16_p5 TCAGTCGTGGTCTTCCAAAC GGAGTCTAGTTCAGCGTCAC 50.0 55.0 57.8 57.7 1262-1263 bp 1.0 77.8