Hotspot

ndhD-psaC

SSC · 122614-122709 · 96 bp

Back to Cyclea

Pi 0.0625
MarkerSeek score 67.80
Primer available yes
Label ndhD-psaC

Feature Metadata

label_name
ndhD-psaC
feature_type
igs
region
SSC
length_bp
96
variable_sites
6
pi
0.0625
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD-psaC_p1 TCGTGGTAAATACAAGATACACT ATGAGACAACTCGGAGCATG 34.8 50.0 55.2 57.7 162 bp 1.0 43.5
ndhD-psaC_p2 TCGTGGTAAATACAAGATACACT CATGAGACAACTCGGAGCAT 34.8 50.0 55.2 57.7 163 bp 1.0 43.4
ndhD-psaC_p3 TCGTGGTAAATACAAGATACACT GGATTTCTTGAGTGTCCGGG 34.8 55.0 55.2 58.3 194 bp 1.0 42.9
ndhD-psaC_p4 ACAAGATACACTTTGACCAT GGATTTCTTGAGTGTCCGGG 35.0 55.0 52.1 58.3 183 bp 0.5 20.0
ndhD-psaC_p5 ACAAGATACACTTTGACCAT ATGAGACAACTCGGAGCATG 35.0 50.0 52.1 57.7 151 bp 0.5 20.0