Hotspot

rpl32-trnL-UAG

SSC · 118562-119629 · 1068 bp

Back to Cyclea

Pi 0.0132
MarkerSeek score 60.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1068
variable_sites
14
pi
0.0132
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GCAGCACTAAACGCTTTCTC TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.8 57.9 1203 bp 1.0 78.3
rpl32-trnL-UAG_p2 CGTTTTGGGCAGCACTAAAC TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.2 57.9 1211 bp 1.0 78.3
rpl32-trnL-UAG_p3 GTTTTGGGCAGCACTAAACG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.2 57.9 1210 bp 1.0 78.3
rpl32-trnL-UAG_p4 GCAGCACTAAACGCTTTCTC TTATAAGATGCCGCCACTCG 50.0 50.0 57.8 57.9 1252 bp 1.0 78.1
rpl32-trnL-UAG_p5 GTTTTGGGCAGCACTAAACG TTATAAGATGCCGCCACTCG 50.0 50.0 58.2 57.9 1259 bp 1.0 78.1