Hotspot

ndhF-rpl32

SSC · 117392-118387 · 996 bp

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Pi 0.0121
MarkerSeek score 62.40
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
996
variable_sites
12
pi
0.0121
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GTCGGAACAAGGAGAAGTCC TACGTTTCTTCGGAACTGCC 55.0 50.0 57.9 58.2 1110-1121 bp 1.0 78.9
ndhF-rpl32_p2 GTCGGAACAAGGAGAAGTCC GAGAAAGCGTTTAGTGCTGC 55.0 50.0 57.9 57.8 1186-1197 bp 1.0 78.6
ndhF-rpl32_p3 GTCGGAACAAGGAGAAGTCC CGTTTAGTGCTGCCCAAAAC 55.0 50.0 57.9 58.2 1179-1190 bp 1.0 78.6
ndhF-rpl32_p4 GTCGGAACAAGGAGAAGTCC GTTTAGTGCTGCCCAAAACG 55.0 50.0 57.9 58.2 1178-1189 bp 1.0 78.6
ndhF-rpl32_p5 GAACAAGGAGAAGTCCCAGC TACGTTTCTTCGGAACTGCC 55.0 50.0 58.2 58.2 1106-1117 bp 1.0 78.0